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| Thesis Name： |
Study on simultaneous determination of sugar and nicotine with high performance liquid chromatography |
| Usage： |
chemistry |
| Keyword： |
high performance liquid chromatography, tobacco, sugar, nicotine |
| Remarks： |
Abstract The simultaneous determination of sugar and nicotine with high performance liquid chromatography (HPLC) in tobacco was studied. The sugar and nicotine was extracted from the tobacco samples by ultrasonic oscillation for 30 min with 45 ml of 5％ acetic acid solution. Then, the extract was purified by solid extraction with a Waters Sep-Pak-C18 cartridge. The sugars and nicotine were separated on a Waters Sugar-Pak-1 (6.5×300 mm) cations ion exchange column with 0.05 g.L-1 EDTA calcium disodium salt solution as mobile phase. The sugar was detected with a refractive index detector and the nicotine was detected with an UV detector. The detection limit reachs m g/ml level for sugar and ng/ml level for nicotine. The recoveries of sugars and nicotine were between 97- 102 %. The relative standard derivations of sugars and nicotine were 1.3-2.2%. This method was applied to the determination of Sugars and nicotine in tobacco with good result. |
| Original file： |
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Promulgator Detail |
| Full name: |
administrator |
| Country: |
CN |
| City: |
Dalian |
| Company Name: |
teloon Soft Strategy Co.,LTD |
| Homepage: |
http://m.hqsq.net |
| E-mail: |
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| Phone: |
86-411-82811662 |
| Fax: |
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| Company Address: |
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| Zip/Postal Code: |
116001 |
| Introduction: |
As a professional E-information service platform with authoritative news and reports in chemical industry of chinese export and import market.Hichem has been received universally and praised highly since its established in 2001.In 2003,by joint effort of teloon chemicals and teloon soft strategy company(TSSC),Hichem team endeavored to recomposition the site ascribed to market requirement as well as offering our better service.Hichem focused on business to biusiness of new chemicals,fine chemicals,high chemicals and special chemicals.So naturally it is the very one-stop website for chemical information.Our sellers worldwide and build global on-line trade in the chemical industry. |
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