| |
| Thesis Name： |
Reaction microarrays and small-molecule printing. |
| Usage： |
chemistry |
| Keyword： |
Reaction microarrays ,small-molecule printing |
| Remarks： |
The development of reaction microarrays, a new technique for the parallelenantiomeric excess measurement of tens-of-thousands of samples, and extensions of this methodologyare presented in this thesis. Inspiration for this work came from a desire to blend thehigh-throughput experimentation of combinatorial catalysis with the high-throughput analysis of DNAmicroarrays. We sought to adapt the high-throughput analytical format of DNA microarray technologyto the measurement of the enantiomeric composition of solutions of small molecules that wereenvisioned to arise from catalytic, enantioselective reactions.; Amino acid derivatives were contactprinted via DNA microarrayers and covalently attached to derivatized glass microscope slides. Twopseudoenantiomeric fluorescent chiral probes, Cy3-(R)-proline and Cy5-(S)-proline, were observed toprovide enantiomeric discrimination of these immobilized amino acids under amide couplingconditions. Kinetic resolution in this amide coupling step provided a ratio of fluorophores at eachprinted spot that was proportional to the enantiomeric ratio of amino acid at that spot. Laserscanning of the glass slides yielded a fluorescence intensity ratio of fluorophores at each printedspot that was used to calculate the enantiomeric excess of the original sample solutions. Theapplicability of the reaction microarray method for high-throughput analysis was demonstrated byrapid identification of two >99% ee samples of proline out of 15,552 total samples.; A generalstrategy for the immobilization of organic small molecules to a glass surface was also developed.Substrates were printed onto sulfonyl azide-derivatized glass slides, which were then photolyzedusing a handheld 365 nm UV lamp. Arrayed substrates were found to be covalently attached to thesurface via a presumed reactive nitrene intermediate. Biologically relevant small molecules,including those not possessing reactive functional groups, were immobilized on a glass surface usingthis photolytic sulfonyl azide decomposition strategy. These arrays were used to detect the bindingof small molecules by proteins.
Author: Korbel, Gregory Alan.
Unit: Harvard University. |
| Original file： |
Download
|
|
Promulgator Detail |
| Full name: |
administrator |
| Country: |
CN |
| City: |
Dalian |
| Company Name: |
teloon Soft Strategy Co.,LTD |
| Homepage: |
http://m.hqsq.net |
| E-mail: |
reply |
| Phone: |
86-411-82811662 |
| Fax: |
86-411-82819667 |
| Company Address: |
26 Renmin Road ,Dalian China |
| Zip/Postal Code: |
116001 |
| Introduction: |
As a professional E-information service platform with authoritative news and reports in chemical industry of chinese export and import market.Hichem has been received universally and praised highly since its established in 2001.In 2003,by joint effort of teloon chemicals and teloon soft strategy company(TSSC),Hichem team endeavored to recomposition the site ascribed to market requirement as well as offering our better service.Hichem focused on business to biusiness of new chemicals,fine chemicals,high chemicals and special chemicals.So naturally it is the very one-stop website for chemical information.Our sellers worldwide and build global on-line trade in the chemical industry. |
|
|
|
|
